目的 建立蛴螬中尿嘧啶、胞苷、次黄嘌呤、黄嘌呤、尿苷、肌苷、鸟苷含量的HPLC方法。方法 采用高效液相色谱法,色谱柱为Waters HSS T3(4.6 mm×250 mm,5 μm),流动相为乙腈(A)和水(B)进行梯度洗脱,检测波长为260 nm,流速为1.0 mL·min-1,柱温为30 ℃。结果 7种成分线性回归方程的相关系数为0.999 1~1.000 0;平均回收率(n=6)在91.2%~97.4%,RSD在1.5%~2.3%。结论 该方法简便、准确、可靠,可为蛴螬的质量控制提供参考。
Abstract
OBJECTIVE To establish a quantitative method for determining the contents of uracil, cytidine, hypoxanthine, xanthine, uridine, inosine, and guanosine in Holotrichia diomphalia Larvae by HPLC. METHODS The HPLC analysis was performed on Waters HSS T3 column(4.6 mm×250 mm, 5 μm). The mobile phase was composed of acetonitrile(A) and water(B), and gradient elution was carried out. The flow rate was 1.0 mL·min-1. The column temperature was maintained at 30 ℃. The detection wavelength was 260 nm. RESULTS The correlation coefficients of the seven components ranged from 0.999 1 to 1.000 0. The average recovery rates(n=6) were between 91.2% and 97.4%, and the RSDs were between 1.5% and 2.3%. CONCLUSION The proposed method is simple, accurate and reliable, thus providing basis for comprehensive quality control of Holotrichia diomphalia Larvae.
关键词
蛴螬 /
尿嘧啶 /
胞苷 /
次黄嘌呤 /
黄嘌呤 /
尿苷 /
肌苷 /
鸟苷 /
含量测定
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Key words
Holotrichia diomphalia Larvae /
uracil /
cytidine /
hypoxanthine /
xanthine /
uridine /
inosine /
guanosine /
content determination
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中图分类号：
R284
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参考文献
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脚注
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基金
国家自然科学基金项目资助(51609100);2017吉林省药监局中药材标准制定修订项目资助(2017065)
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